Composite

Part:BBa_K5250014:Design

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-10-01)


Prha + PisoF_0056 DGC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 558
    Illegal PstI site found at 627
    Illegal PstI site found at 845
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 558
    Illegal PstI site found at 627
    Illegal PstI site found at 845
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 278
    Illegal BamHI site found at 1176
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 558
    Illegal PstI site found at 627
    Illegal PstI site found at 845
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 558
    Illegal PstI site found at 627
    Illegal PstI site found at 845
    Illegal NgoMIV site found at 866
    Illegal NgoMIV site found at 989
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The parts have been codon optimized.


Source

The DGC sequence we obtained from a Pseudomonas species IsoF gene bank our host lab provided us with. The rest of the parts come from the iGEM Parts registry.

References